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FAQ (frequently asked questions)
- What is the benefit of Bio-Nobile’s technology for me?
- What is the throughput with different devices?
- What kind of samples can I purify using Bio-Nobile’s solutions?
- More questions, by subject area
BN offers innovative products allowing easy and fast purification of samples for
your downstream applications such as PCR. Purification of nucleic acids such as
gDNA, Plant gDNA, plasmid DNA, DNA fragment and mRNA as well as
purification/fractionation of proteins such as weak ion exchangers, IMAC and GST
becomes far more straightforward with our PickPen® magnetic tools and range of
QuickPick™ kits in manual and automation processes.
All of the QuickPick kits are optimized for use with manual PickPen magnetic
tools and most of them can be automated. The kits are for research use only.
The
MagRo™ 8-M robotic workstation integrates magnetic particle processing and
liquid handling in one flexible instrument. This versatility enables the
dispensation of samples and reagents followed by the purification step in a
single process. MagRo 8-M processes 8 wells at a time in batch sizes of up to 96
samples per run and up to 300 samples per day depending on the application.
The PickPen 1-M magnetic tool is designed to purify a few sample per day in
vessels such as microtubes, bottles etc.
The PickPen 8-M magnetic tool offers the same benefits as the PickPen 1-M but
allows parallel processing of 8 samples. The device is suitable for use with
QuickPick kits in both standard and deep well microplate applications.
The QuickPick gDNA purification kits are intended for universal genomic
DNA purification from various sample materials. The Sample amount for
purification is from 1-200 µl.
The QuickPick Plant DNA kits are for purifying genomic DNA from plant
tissues such as leaf, root, seed etc. The Sample amount for purification is from
1-100 mg.
The QuickPick DNA Fragment kits provide a fast and simple means of
purifying 60 bp to 50 kbp DNA fragments from agarose gel or from liquid samples.
The kit is suitable for use with standard and low-melting agarose gels in TAE
buffer.
The QuickPick Plasmid DNA purification kits are used to purify high-,
medium- and low-copy plasmids from bacteria cells. Typical culture volume is 1.5
ml but it can be scaled up.
The QuickPick mRNA kits are intended for mRNA purification from various
sample materials such as cultured cells, animal and plant tissue and total RNA
samples. There are four QuickPick™ kit versions available to accommodate
purifications from starting materials containing just one cell to approximately
300 mg of tissue.
For protein purifications there are fractionation kits such as QuickPick DEAE
(using weak anion exchange) and QuickPick CM (using weak cation exchange)
and affinity-based kits such as QuickPick IMAC (histidine) and
QuickPick GST (glutathione-S-transferase) for fusion proteins. The IMAC kit
can be used to purify up to 0.5 mg protein per prep, and at least 40 µg of
GST-fusion protein can be purified per prep.
- General questions about PickPen technology
- Frequently asked questions about manual
devices
- General questions about automation
- General questions about automation processes
- Frequently asked questions about nucleic acid purifications
- Frequently asked questions about protein purifications
- Frequently asked questions about other solutions
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