|
General questions about PickPen®
technology
- How does the PickPen® technology differ from other purification technologies?
- What kind of devices does Bio-Nobile offer for its PickPen technology?
- What is the capacity of PickPen to collect magnetic particles?
- What kind of magnetic particles do you have?
- What is the binding capacity of magnetic particles?
- What is the composition of magnetic particles?
- What is the size of magnetic particles?
The Bio-Nobile’s PickPen® method principle “moving magnetic particles, not
liquids” offers a clear advantage to the conventional purification methods.
Using PickPen technology, your nucleic acid and protein sample purifications
are performed faster, simpler and gentler and are ready for downstream
applications such as PCR.
Unlike other commercial magnetic bioseparation systems, which normally rely on
fixed external magnets and liquid transfer to achieve separation, Bio-Nobile's
innovative hand-held PickPen devices as well as MagRo 8-M robotic workstation
move magnetic particles with bound target molecule.
The original version of the PickPen magnetic tool is a hand-held single-magnet
device PickPen 1-M. You can use it to carry the sample yourself from one stage
of the assay to the next. It is possible to observe the MPs transfer from one
vessel to another.
There is also an 8-magnet manual version, PickPen 8-M, which allows you to
process samples in a microplate format one whole column at a time.
Bio-Nobile offers also full automation for medium to high throughput sample
purification and liquid handling, MagRo™ 8-M robotic workstation. MagRo 8-M
processes 8 wells at a time with a capacity of up to 96 samples per run.
The collection capacity depends on the physical nature (size, amount of
magnetite etc.) of the particular magnetic particle. Typically, PickPen can
collect at least 500 mg of magnetic particles from a solution.
We have magnetic particles for different applications, for example, gDNA, DNA
fragment, mRNA, IMAC (histidine-tagged proteins), GST fusion proteins and weak
ion exchangers. Information on kits based on these particles is found on our
product pages. Full details of other particles are available
on our coated particles page.
Binding capacity depends on the magnetic particle. For example 5 µl of Magazorb™
Magnetic Particles can bind 125 µg of DNA in optimum conditions. Separately sold
magnetic particles will be delivered with their own specifications, and the
amount used is based on the customer downstream application.
Magnetic particles are paramagnetic, i.e., they react to a magnetic field
without themselves becoming magnetized. Consequently, once they are removed from
the magnetic field, paramagnetic particles cease to bond together, and can
readily be resuspended.
There are various kinds of particles available. They usually have a core of iron
and are bonded or coated with silane, glass, or various polymers. The relevant
information will usually be specified for the specific magnetic particle.
The surface of the particles has usually some chemical groups or molecules which
will bind the target substance specifically. Particles may be classified on the
basis of their surface coating material. There are particles with simple surface
groups (e.g. -NH2), with general recognition groups (e.g. oligo-dT) and with
specific recognition groups (e.g. monoclonal antibodies).
This depends on the type of the magnetic particle. They are generally 1-10 µm in
diameter.
|
