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QuickPick solutions for nucleic acid purification
Bio-Nobile offers a range of QuickPick kits for
plant DNA,
genomic DNA, DNA fragment,
plasmid DNA and mRNA purifications. QuickPick nucleic acid kits are optimized
for use with PickPen® 1-M and PickPen 8-M magnetic tools as well as with MagRo
8-M robotic workstation.
Benefits of QuickPick DNA purification
Scalable and Flexible
- easy solution for variable sample volumes
- use with PickPen magnetic tools or the MagRo workstation
Fast purification
- rapid protocols ensure high-quality DNA
- short hands-on-time
Reliable
- reproducible results
- robust protocols
- high-quality purified product
Benefits of QuickPick mRNA purification
Scalable and Flexible
- wide range of sample materials
- scalability up to 1 x 106 cells
- use with PickPen® magnetic tools or the MagRo
workstation
Fast purification
- 96 samples in one hour
- Suitable for downstream applications without elution
Reliable
- reproducible results
- robust protocols
- high-quality purified mRNA
Wide range of QuickPick product formats
QuickPick Ready-to-use kits
QuickPick DNA ready-to-use kits are optimized for constant sample
volumes to be used with the PickPen 1-M and PickPen 8-M magnetic tools. These
ready-to-use kits contain reagents and consumables needed for the purification.
Plant DNA and plasmid DNA kits are available in three
kit sizes: 8, 48 and 96 preparations. DNA Fragment kit is available in 24 and
3x96 preparations.
QuickPick Scalable kits
QuickPick DNA and mRNA scalable kits can be used for variable sample sizes. The sample
volumes may be scaled up and down, giving the yield of high-quality
product appropriate for the intended downstream application.
QuickPick Bulk reagents
QuickPick DNA and mRNA reagents are also available for custom and bulk configurations.
Bio-Nobile Plant DNA purification solutions
Purify DNA from a comprehensive range of plant species with QuickPick Plant
DNA reagents. High quality DNA can be extracted from different sample materials eg. leaf, seed or root.
DNA purified using QuickPick Plant DNA reagents typically show an approximate
size of 20-30 kbp which is suitable for several downstream applications such as
PCR, sequencing, RE digestion and cloning. Increasing the sample amount will
lead to increasing yield.
| Sample |
Amount |
Yield, µg |
Purity, 260/280* |
| Hordeum Vulgare (barley) |
50 mg |
6.0 |
≥ 1.8 |
| Lactuca sativa L. c.v. Grand Rapids |
50 mg |
3.0 |
≥ 1.8 |
| Ocimum basilicum (basil) |
50 mg |
5.0 |
≥ 1.8 |
| Arabidopsis Thaliana |
50 mg |
2.0 |
≥ 1.8 |
| *Ratio of absorbance at 260/280 nm corrected
with absorbance at 320 nm |
Kit specifications for QuickPick Plant DNA purification reagents
| Starting material amount |
25 mg |
50 mg |
100 mg |
 |
| Typical DNA yield* |
Up to 3 µg |
Up to 6 µg |
Up to 12 µg |
| Typical purity** |
≥ 1.8 |
| Size of purified DNA |
≥ 30 Kb |
*DNA yield varies greatly between different kinds of sample material.
Purifications performed using barley as a sample material.
**Ratio of absorbance at 260/280 nm corrected with absorbance at 320 nm |
Download QuickPick Plant DNA
purification protocol
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Bio-Nobile gDNA purification solutions
The QuickPick gDNA kit is universal and suitable for purifying genomic DNA from
a wide variety of starting materials such as human and animal blood, buffy coat,
leukocytes, hair, saliva, cultured cells, tissue, swap, buccal cells, urine and
bacteria. DNA purified using QuickPick gDNA reagents show a recovery of at least 30 kbp
which is suitable for all typical downstream applications, including: PCR,
sequencing, RE digestion and cloning.
Specification of the QuickPick gDNA purification kit,
using the MagRo robotic workstation
| Sample |
Amount |
Yield |
Amount |
Yield |
Amount |
Yield |
|
| Whole Blood |
50 µl |
Up to
1.5 µg |
100 µl |
Up to 3 µg |
200 µl |
Up to 6 µg |
| Buffy Coat |
50 µl |
Up to
3 µg |
100 µl |
Up to 6 µg |
200 µl |
n.d. |
| Leukocytes |
0.5 million |
Up to
4 µg |
1 million |
Up to 8 µg |
2 million |
n.d. |
| Cultured Cells |
1 million |
Up to
2.5 µg |
2 million |
Up to 5 µg |
4 million |
Up to 10 µg |
| Tissue (liver) |
2.5 mg |
Up to 8 µg |
5 mg |
Up to 16 µg |
10 mg |
Up to 32 µg |
| Hair |
2 - 4
strands |
Up to
0.5 µg |
6 - 8
strands |
Up to 1 µg |
12 - 16
strands |
n.d. |
| Typical purity |
≥ 1.7 |
| Size of purified DNA |
≥ 30 kbp |
| |
Yields will depend on species, growth
conditions, etc.
Leukocytes were suspended in 50-200 µl of PBS
depending on sample amount
Ratio of absorbance at 260/280
nm is corrected with absorbance at 320 nm
n.d. = not determined |
Download QuickPick gDNA purification
protocol
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Bio-Nobile DNA Fragment purification solutions
Use QuickPick DNA Fragment kit for simple and quick purification of DNA
fragments from gel or liquid solutions. The kit works well both with standard
and low-melting agarose gels in TAE and TBE buffer. There is no limit for the
weight of the gel piece in the manual protocol.
Ethanol traces in the elution buffer is a common problem in DNA fragment
purification methods. A unique and proprietary DipWash method made possible by
the PickPen technology is used in the QuickPick purification procedure to
eliminate this problem. During the DipWash process the magnetic particles are
not released from the PickPen tool, instead a few dips in the Wash Buffer is
enough to rinse the ethanol away. The method is rapid and easy to use and
guarantees the recovery of high quality DNA.
Specifications of the QuickPick DNA Fragment kit
| DNA fragment size in agarose gel |
60 bp 50 kbp |
| Gel type |
Any type of agarose gel |
| Buffer type |
TAE or TBE buffer |
| Weight of gel piece / one prep |
100 ± 20 mg |
| Recovery (60 bp 50 kbp) |
> 80 % |
| Binding capacity / preparation |
10 µg |
| Typical purity* |
≥1.8 |
| Elution volume |
5 100 µl |
| Total protocol time |
< 15 min |
| *Ratio of absorbance at 260/280 nm corrected with absorbance at 320 nm |
Download QuickPick DNA Fragment purification protocol
Excision of DNA fragments from agarose gels
Bio-Nobile offers an innovative method for excising DNA fragment from gel by
using its PickO gel exciser. The method is a one-hand process with high
efficiency, each piece from the same gel is the same size. No
extra weighing is required.
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Bio-Nobile Plasmid DNA purification solutions
QuickPick Plasmid DNA kits provide fast and simple purification of plasmid DNA
from bacterial cells. The purification protocol is based on a modified alkaline
lysis procedure followed by magnetic purification of the plasmid. The plasmid
DNA purified by the QuickPick Plasmid DNA kit is ready for downstream
applications such as DNA sequencing, transformation, PCR and cloning.
M 1 2
3 4 5
6 7 8
|
 |
Plasmid DNA isolated with the QuickPick Plasmid DNA kit from E.
coli XL1-Blue culture, and with analysis on 1% agarose gel. M = marker,
1 - 8 = Parallel samples of plasmid DNA isolated from 1.5 ml of E.coli culture
Specifications of the
QuickPick Plasmid DNA kit
| Vessel format |
1.5 ml microtubes, 96-well microplates |
| Starting material per preparation |
1.5 ml E. coli
cells
(OD600 = 3-4) |
| Elution volume |
5-100 μl |
| Yield per preparation |
Up to 11 μg |
| Purity* |
> 1.8 |
| * Ratio of absorbance at 260/280 nm corrected with absorbance at 320 nm |
Download QuickPick Plasmid DNA purification protocol
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Bio-Nobile mRNA purification solutions
Using QuickPick SML mRNA kits, you can enjoy all of the benefits of the
PickPen® method and purify high quality mRNA directly in a rapid 10-minute
protocol. QuickPick SML mRNA purification kits provide a fast and simple means
of purifying poly-A(+) mRNA from cells, total RNA and animal or plant tissues.
The purified mRNA can be used for down-stream applications such as RT-PCR,
real-time PCR, cDNA libraries and microarray analyses.
100% recovery is obtained by using solid phase elution and amplifying mRNA
directly from magnetic particles suspended in suitable buffer.
Specifications of the QuickPick
SML mRNA kit
| Sample |
Amount |
Amount |
Amount |
|
| Cells |
≤ 103 cells |
103
- 105 cells |
105
- 106 cells |
| Animal tissue |
≤ 30 µg |
30 µg - 3 mg |
3 - 30 mg |
| Plant tissue |
≤ 50 µg |
50 µg - 5 mg |
5 - 50 mg |
| Total RNA |
≤ 50 ng |
50 ng
- 5 µg |
5 - 50 µg |
|
Typical purity |
Applicable for RT-PCR |
Download QuickPick
SML mRNA purification protocol
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